学术报告：SILICON SURFACE CHEMISTRY FOR BIOMEDICAL APPLICATIONS
报告题目：SILICON SURFACE CHEMISTRY FOR BIOMEDICAL APPLICATIONS报告人： Shou-Jun Xiao（State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, School of Chemistry and Chemical Engineering, Nanjing University）
时间： 9月13日（周二）下午1点
地点： 嘉定园区学术活动中心302室
Silicon (Si) has been predicted to become capital in biomedical technology since many years ago. And there is growing interest and rapid development in the surface chemistry of Si for attaching soft materials and biological molecules. This is not only because of its ubiquitous presence in microelectronics and microfabrications, but also its unique properties of the Si-H bonds on the surface, especially their reactivities towards different organic and organometallic molecules. Porous silicon (PSi) has rich physical and chemical properties such as large internal surface area (hundreds of m2/cm3) for high loading of biomolecules, significant semiconductor properties for micro- and nano-fabrication and for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, indicative photonic crystal behavior for interference fringe analysis with light reflection, transparency to infrared light for convenient IR analyses, and low fluorescence background after solvent treatment for fluorescence imaging and evaluation.
To address the surface chemistry of silicon, we developed a Multiple Transmission-Reflection Infrared Spectroscopy (MTR-IR), which has the same sensitivity as the Multiple Internal Reflection Infrared Spectroscopy (MIR-IR) but is much convenient to use. By means of infrared spectroscopy, we figured out the secret of two well-accepted reaction mechanisms. It tells that stepwise surface chemistries need careful attention since they are similar to bulk chemistries: significant difference exists in side products and reaction efficiencies even for analogous molecules. The IMAC (immobilized metal ion affinity chromatography) chemistry, NiII–NTA (nitrilotriacetic acid)/histidine-tagged protein, is carried out on silicon surfaces for chelation-based biochips. The grafted protein was detected and analyzed by cross-checking methods: MALDI-TOF-MS, fluorescence scanning and AFM imaging. By means of polymer brushes grown from silicon surfaces by atom transfer radical polymerization (ATRP), higher loadings of probe molecules and thus target proteins can be realized. Silicon-based DNA nanotechnology is also under investigation.
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