报告题目：Interfacing DNA with metal ions, metal oxides, and metal nanoparticles

报 告 人：刘珏文

报告时间：2015年6月5日（星期五）上午10：30

报告地点：嘉定园区学术活动中心一楼多功能厅

报告简介：

Abstract: DNA is a polyanion with strong affinity towards metal containing species. We are interested in studying the interaction between DNA and metal for analytical applications. This talk will cover three aspects. 1) In vitro selection of metal-specific DNAzymes. DNAzymes are DNA-based catalysts and we focus on RNA-cleaving DNAzymes. Using lanthanide ions as cofactors, we isolated a suite of new DNAzymes that can selectively detect rare-earth metals. By using phosphorothioate modified DNAzymes, we developed new probes for a few toxic heavy metal ions. 2) The adsorption of DNA by various metal oxides were studied, where the phosphate backbone of DNA is shown to be critical for the adsorption reaction. Using fluorescently labeled DNA, highly sensitive detection of arsenate and hydrogen peroxide was achieved. 3) DNA adsorption by gold nanoparticles is studied as a function of salt concentration and pH. Based on this study, we developed an ultrasensitive method for functionalizing gold surface with DNA.

报告人简介：

刘珏文，加拿大滑铁卢大学化学系副教授，博士生导师。2000年毕业于中国科技大学化学系，获得本科学位。2005年获美国伊利诺伊大学(UIUC)化学系博士学位（导师Yi Lu教授），课题方向为脱氧核酸酶在金属离子检测中的应用。2005-2007年继续在Dr. Yi Lu实验室进行博士后工作。2007-2009年在美国新墨西哥大学和Sandia国家实验室进行基于介孔氧化硅的药物传递系统的博士后研究工作（导师 C. Jeffrey Brinker教授, 美国工程院院士）。从2009年起受聘于加拿大滑铁卢大学。在过去六年内，获得加拿大自然科学基金，加拿大创新基金，加拿大化学会Fred Beamish奖，安大略省青年研究者奖等多项研究资助。近年来接收和发表期刊研究论文和书籍章节共计130余篇，其中Acc. Chem. Res. (1), Chem. Rev. (1), PNAS (2), J. Am. Chem. Soc. (18), Angew. Chem. Int. Ed. (5), Adv. Mater. (3), Anal. Chem. (13), 引用数达6000余次。其中引用超过100次的有20篇。担任多个杂志的审稿工作。现主要从事核酸适配体，纳米材料和软物质的分析化学，物理化学，以及表面性质的研究。